Creatine kinase: ATP contaminating creatine phosphate substrate of a commercial kit.

The Effect of Cigarette Smoking on Human Sperm Creatine Kinase Activity: As An ATP Buffering System in Sperm

Compartmentation of mitochondrial creatine phosphokinase. I. Direct demonstration of compartmentation with the use of labeled precursors.

Mitochondrial creatine kinase was first proposed to act as a functional component in respiratory control in 1966 (Bessman, S. P., and Fonyo, A. (1966) Biochem. Biophys. Res. Commun. 22, 597-602). Since that time, evidence has accumulated to support the theory of a creatine-phosphorylcreatine shuttle mechanism involved in supplying energy for aerobic muscle contraction (Bessman, S. P., and Geige...

Effect of creatine kinase activity on mitochondrial ADP/ATP transport. Evidence for a functional interaction.

Mitochondrial adenine nucleotide translocation was measured in the presence of creatine or creatine phosphate to test the proposed functional couple between the nucleotide translocase and creatine kinase. Rat heart mitochondria were preloaded with the corresponding nucleotide which reacts with creatine kinase only after it is transported across the mitochondrial membrane; namely, radioactive AT...

In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility

Background Nicotine, cotinine and cadmium are harmful components of cigarettes that have an effect on human reproductive function. Although the effects of cigarette smoke on male reproductive function is characterized in several articles its mechanism of action is still unknown. In the present study, we investigate the effect of nicotine, cotinine and cadmium on human sperm creatine kinase acti...

Metal and metal-ATP interactions with brain and cardiac adenylate cyclases.

Metal (Me) and MeATP interactions with adenylate cyclases associated with rabbit ventricular particles and with a detergent-dispersed preparation from rat cerebellum have been studied. data were simulated to fit kinetic models in which an inhibitor (HATP or ATP) is added in constant proportion to the variable substrate (MeATP). The specific models considered were that the enzyme binds (a) MeATP...